Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli beneath the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations
We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and 3.12 U/mgprotein of transglutaminase exercise have been obtained. DO-stat fed-batch cultivations beneath the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein).
DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose beneath the identical circumstances produced even increased enzymatic exercise (9.14 U/mgprotein). The pH impact was investigated, and the very best enzymatic exercise could possibly be noticed at pH 8. In all cultivations, the bicistronic system remained steady, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to precise recombinant TGase could possibly be cultivated in bioreactors beneath DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this essential enzyme.
Description: Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kDa which comprises the receptor binding domain of the Mpl-ligand protein.;The TPO is purified by proprietary chromatographic techniques.
Description: Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.;The TPO is purified by proprietary chromatographic techniques.
Description: Thrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton._x000D_ The TPO is purified by proprietary chromatographic techniques. _x000D_
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Thrombopoietin (TPO)Serum, plasma and other biological fluids
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Thrombopoietin, TPO in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Thrombopoietin (TPO) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Description: TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Description: TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
TPO Human, ThrombopoietinHuman Recombinant Protein, His Tag
Description: TPO produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 340 amino acids (22-353aa.) andhaving a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57KDa).;TPO is expressed with an 8 amino acid His tag at C-Terminusand purified by proprietary chromatographic techniques..
Description: Quantitativesandwich ELISA kit for measuring Rat Thrombopoietin, TPO in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Thrombopoietin, TPO in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Dog Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Dog Thrombopoietin (TPO) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Thrombopoietin (TPO) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitativesandwich ELISA kit for measuring Mouse Thrombopoietin, TPO in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Thrombopoietin, TPO in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Thrombopoietin (TPO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Thrombopoietin (TPO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Thrombopoietin (TPO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Thrombopoietin (TPO) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Thrombopoietin (TPO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Canine Thrombopoietin (TPO) in samples from serum, plasma or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Thrombopoietin (TPO) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Thrombopoietin (TPO) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Thrombopoietin/TPO in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich ELISA kit for detection of Thrombopoietin from Dog in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Thrombopoietin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Thrombopoietin from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Recombinant Human Thrombopoietin/ THPO Protein, Untagged, E.coli-500ug
Recombinant Protein Manufacturing and Purification Utilizing Eukaryotic Cell Factories
Cloning proteins allows their manufacturing and characterization for additional research. This requires inserting the gene of the studied protein to be inserted in a vector, which then shall be remodeled to the host cell used as “manufacturing unit.” Consequently, the “biomass” of host cells shall be produced utilizing bioreactors. Right here we describe the manufacturing of Rhizomucor miehei lipase (RML) by cloning the corresponding genes within the yeast Pichia pastoris. This enzyme is used as a biocatalyst for biofuel manufacturing. The efficiently produced recombinant proteins are then purified utilizing ion change chromatography.
Modulating Oral Supply and Gastrointestinal Kinetics of Recombinant Proteins by way of Engineered Fungi
A brand new modality in microbe-mediated drug supply has lately emerged whereby genetically engineered microbes are used to domestically ship recombinant therapeutic proteins to the gastrointestinal tract. These engineered microbes are also known as reside biotherapeutic merchandise (LBPs). Regardless of superior genetic engineering and recombinant protein expression approaches, little is understood on learn how to management the spatiotemporal dynamics of LBPs and their secreted therapeutics inside the gastrointestinal tract.
To this point, the elemental pharmacokinetic analyses for microbe-mediated drug supply methods haven’t been described. Right here, we discover the pharmacokinetics of an engineered, mannequin protein-secreting Saccharomyces cerevisiae, which serves as a perfect organism for the oral supply of advanced, post-translationally modified proteins. We set up three strategies to modulate the pharmacokinetics of an engineered, recombinant protein-secreting fungi system: (i) altering oral dose of engineered fungi, (ii) co-administering antibiotics, and (iii) altering recombinant protein secretion titer. Our findings set up the elementary pharmacokinetics which shall be important in controlling downstream therapeutic response for this new supply modality.
Recombinant human insulin-like progress factor-1 promotes osteoclast formation and accelerates orthodontic tooth motion in rats
Background: IGF-1 could also be an essential consider bone transforming, however its mechanism of motion on osteoclasts throughout orthodontic tooth motion is advanced and unclear.
Methodology: The closed-coil spring was positioned between the left maxillary first molar and higher incisors with a drive of 50 g to determine an orthodontic motion mannequin. Eighty SD rats have been randomized to obtain phosphate buffer saline or 400 ng rhIGF-1 within the lateral buccal mucosa of the left maxillary first molar each two days. Tissue sections have been stained for tartrate-resistant acidic phosphatase (TRAP), the variety of TRAP-positive cells was estimated and tooth motion measured.
Outcomes: The rhIGF-1 group exhibited evidential bone resorption and lacuna appeared on the alveolar bone in comparison with the management group. Furthermore, the variety of osteoclasts in compression aspect of the periodontal ligament within the rhIGF-1 group peaked at day 4 (11.37±0.95 in comparison with 5.28±0.47 within the management group) after the orthodontic drive was utilized and was considerably increased than that of the management group (p<0.01). Moreover, the space of tooth motion within the rhIGF-1 group was considerably bigger than that of the management group from day Four to day 14 (p<0.01), suggesting that rhIGF-1 accelerated orthodontic tooth motion.
Conclusion: Our research has confirmed that rhIGF-1 may stimulate the formation of osteoclasts within the periodontal ligament, and speed up bone transforming and orthodontic tooth motion.
Als3-Th-cell-epitopes plus the novel mixed adjuvants of CpG, MDP, and FIA synergistically enhanced the immune response of recombinant TRAP derived from Staphylococcus aureus in mice
Introduction: Staphylococcus aureus (S. aureus) is a gram-positive opportunistic pathogen, there are at the moment no excessive efficient vaccine in opposition to S. aureus in people and animals, the event of an environment friendly vaccine stays an essential problem to stop S. aureus an infection. Right here, we ready Als3-Th-cell-epitope-Goal of RNAIII Activating Protein (TRAP) (ATT) proteins plus the novel mixed adjuvants to develop a promising vaccine candidate in opposition to S. aureus.
Strategies: The recombinant pET-28a (+)-att plasmids have been constructed, and the ATT proteins have been expressed and obtained, then, ATT plus Freund’s adjuvant or the novel mixed adjuvants of cytosine-phosphate-guanosine oligodeoxynucleotides (CpG), muramyl dipeptides (MDP), and FIA have been immunized in mice. After booster immunization, the degrees of interferon-γ (IFN-γ), interleukin-4 (IL-4), IL-10 and IL-17A cytokine have been evaluated, the humoral immune responses in opposition to TRAP have been detected in mice, and the survival fee of mice was confirmed by problem assay.
Outcomes: The mice immunized with ATT plus Freund’s adjuvant exhibited considerably increased stage of IFN-γ, IL-4, IL-10, and IL-17A, and displayed the stronger humoral immune response in opposition to TRAP than management teams, importantly, the survival fee of those mice was considerably increased than management teams. As well as, in contrast with the management teams, ATT + CpG + MDP + FIA group was elicited considerably increased stage of IFN-γ, IL-4, IL-10, and IL-17A and was triggered the stronger humoral immune responses in opposition to TRAP, furthermore, generated the upper survival fee of mice.
Conclusion: Als3 epitopes considerably enhanced TRAP immunogenicity. ATT plus the novel mixed adjuvants of CpG, MDP, and FIA induced the sturdy immune response and safety in opposition to S. aureus, revealing the mix of CpG, MDP, and FIA adjuvant acts the synergistic impact.
Enzymatic Properties of Recombinant Ligase Butelase-1 and Its Software in Cyclizing Meals-Derived Angiotensin I-Changing Enzyme Inhibitory Peptides
Butelase-1 is an environment friendly ligase from Clitoria ternatea with vast purposes within the meals and biopharmaceutical fields. This analysis aimed to realize high-efficiency expression of butelase-1 and discover its software in food-derived angiotensin I-converting enzyme (ACE) inhibitory peptides. The recombinant butelase-1 zymogen was ready at a yield of 100 mg/L in Escherichia coli and efficiently activated at pH 4.5, leading to a 6973.Eight U/L yield of activated butelase-1 with a particular exercise of 348.69 U/mg and a catalytic effectivity of 9956 M-1 s-1. Activated butelase-1 exhibited appreciable resistance to Tween-20, Triton X-100, and methanol.
The “traceless” cyclization of ACE inhibitory peptides was realized utilizing activated butelase-1, which resulted in increased stability and ACE inhibitory exercise than these of the linear peptides. Our work proposed an environment friendly technique for the preparation of butelase-1 and offered a promising mannequin for its software in meals fields.
Description: UPK3A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 214 amino acids (19-207 a.a.) and having a molecular mass of 23.1kDa.;UPK3A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: p53 Human Recombinant full length produced in E.Coli is a non-glycosylated, polypeptide chain having a total Mw of 81kDa. p53 Human Recombinant is fused to GST tag and purified by proprietary chromatographic techniques.
Description: Bcl2 antagonist of cell death (BAD) Human Recombinant full length protein expressed in E.coli, shows a 51 kDa band on SDS-PAGE(Icluding GST tag). ;The BAD protein is purified by proprietary chromatographic techniques.
Description: PHF11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-331 a.a.) and having a molecular mass of 39.7kDa.;PHF11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CTAG2 Recombinant Protein (Human) (Recombinant-P Tag)
Description: KIN Human Recombinant produced in E. coli is a single polypeptide chain containing 416 amino acids (1-393) and having a molecular mass of 47.8 kDa.;KIN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: CD5 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 371 amino acids (25-372) and having a molecular mass of 41.0 kDa.;CD5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: CD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 209 amino acids (25-209 a.a) and having a molecular mass of 23.8kDa.;CD2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: DBH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 599 amino acids (40-617 a.a) and having a molecular mass of 67.2kDa.;DBH is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: CD7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (26-180 a.a) and having a molecular mass of 18.8 kDa.;CD7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ; 800x600
Description: CD9 Human Recombinant produced in E. coli is a single polypeptide chain containing 107 amino acids (112-195) and having a molecular mass of 12kDa.;CD9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor at the early stages of hematopoiesis. SCF promotes the survival, differentiation, and mobilization of myeloid, erythroid, megakaryocytic, lymphoid and melanocyte progenitors
Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor at the early stages of hematopoiesis. SCF promotes the survival, differentiation, and mobilization of myeloid, erythroid, megakaryocytic, lymphoid and melanocyte progenitors
Description: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor at the early stages of hematopoiesis. SCF promotes the survival, differentiation, and mobilization of myeloid, erythroid, megakaryocytic, lymphoid and melanocyte progenitors
Description: Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF), is a membrane-bound glycoprotein produced in the liver, kidney and skeletal muscle. TPO plays important roles in megakaryocytopoiesis and thrombopoiesis.
Description: Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF), is a membrane-bound glycoprotein produced in the liver, kidney and skeletal muscle. TPO plays important roles in megakaryocytopoiesis and thrombopoiesis.
Description: Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF), is a membrane-bound glycoprotein produced in the liver, kidney and skeletal muscle. TPO plays important roles in megakaryocytopoiesis and thrombopoiesis.
Description: QKI Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 364 amino acids (1-341 a.a) and having a molecular mass of 40.1kDa.;QKI is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: SET Human Recombinant produced in E. coli is a single polypeptide chain containing 313 amino acids (1-290) and having a molecular mass of 35.9kDa. SET is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: C1D Human Recombinant produced in E. coli is a single polypeptide chain containing 164 amino acids (1-141) and having a molecular mass of 18.4kDa. C1D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
